The rise of Vancomycin-Resistant Staphylococcus aureus (VRSA) among Methicillin-Resistant Staphylococcus aureus (MRSA) strains poses a significant challenge in the medical field. Glycopeptides such as vancomycin are frequently the antibiotics of choice for the treatment of infections caused by methicillin resistant Staphylococcus aureus (MRSA). For the last few years incidence of vancomycin intermediate S. aureus and vancomycin resistant S. aureus (VISA and VRSA respectively) has been increasing in various parts of the world. Accurate estimation of the Minimum Inhibitory Concentration (MIC) is essential for confirming VRSA and guiding appropriate therapy. This study compares the accuracy and usefulness of two popular MIC determination techniques: agar dilution and the E-test strip method. AIM AND OBJECTIVE: A comparative study between agar dilution method and e-test strip method for the determination of minimum inhibitory concentration (MIC) to confirm VRSA among MRSA. MATERIAL AND METHODS: This was a observational Cross sectional study carried out in the Department of Microbiology at Government Medical College, Kota Rajasthan. The samples were processed immediately reaching the lab, in case of delay the samples were refrigerated at 40C. A total of 185 clinical isolates of MRSA were screened out of 384 Staphylococcus aureus isolates which were tested for vancomycin MICs using both agar dilution and E-test strip methods according to the CLSI guidelines.