In the present study would assess the Physiological variable differences such as breath holding capacity among Physical Education students and Yoga Students who studied in Chaudhary Bansi Lal University, Bhiwani, Haryana. The total number of subjects for this study were 80 students. (40 Physical Education students and 40 Yoga Students). The data for this proposed study were collected from Department of Physical Education and Sports, Chaudhary Bansi Lal University, Bhiwani, Haryana. The data was collected by use of Stop watch and Nose clipper. The data was analyzed and compared with the help of statistical procedures in which arithmetic mean, standard deviation (S.D.), t-test were employed. Physical Education students and Yoga Students breath holding capacity was found no significantly Difference.