This study aims at understanding some important aspects of the antidiabetic properties of Debregeasia longifolia, traditionally recognized for its medicinal efficacy, particularly in ethnic communities where various parts of the plant, especially the roots, have been utilized for their therapeutic benefits over time. Extracts from different parts of the plant have been considered for this study, with a dot blot assay performed to assess their activity comprehensively. The evaluation of anti-diabetic properties has been conducted using a colorimeter and light emitting reaction, with the degree of absorbance serving as the primary indicator of significant changes. The results suggest that not only leaf and bark extracts but also the roots of Debregeasia longifolia exhibit promising antidiabetic potential. Furthermore, the study provides insights into the composition and nutritional values of this plant, highlighting its functional utilization in traditional medicine.