The DNA isolation process started with the selection of twenty different genotypes of okra. However, due to the presence of a high concentration of acidic polysaccharides (mucilage) and polyphenols in okra, it becomes problematic during DNA extraction. For the collection of superior-quality genomic DNA, healthy and juvenile leaves from okra genotypes were collected and rinsed thoroughly with distilled water. Thereafter, a cost-effective, swift, and modest technique was standardized for DNA isolation during the study. Hence, the extracted DNA through the modified method reported a significantly higher yield (20-30 percent more than other methods) and better quality than the traditional methods and was sufficiently pure for approximately 275-300 PCR reactions to study subsequent genetic diversity.