Background: The Rhododendron griffithianum have been used in traditional medicine for the treatment of inflammatory conditions, pain, gastro-intestinal disorders, common cold, asthma, skin disease, etc. Purpose: The focus of this present research is based on the analytical methodologies, which include the isolation of active ingredients from Rhododendron griffithianum. This study focused on isolation and characterization of Rhododendron griffithianum active constituent by column chromatography. Methodology: Various solvent at different polarity were pre-tested in TLC for developing solvent system. The eluted fractions were run in TLC mobile phase with the solvent Toluene: Ethyl acetate: Acetic acid (8:2:0.4). The isolated compounds were characterized by modern analytical techniques such as UV-visible Spectroscopy, FTIR (Fourier Transform Infrared spectroscopy), NMR Spectroscopy and Mass spectroscopy. Results: Spectral analysis confirmed, the existence of coumarin in Fraction A of RGE (F3). More studies are needed in the same lines to evaluate other phytoconstituents of this plant.