Diabetes mellitus, a metabolic disorder is responsible for the death of millions of people across the globe. Poor efficacy, high cost and adverse side effects associated with available synthetic anti-diabetic drugs have necessitate the need to search for antidiabetic drugs of natural origin. Therefore, in this study, anti-diabetic and antioxidant activities of chloroform fraction of Helicteres isora were evaluated. Phytochemical composition, in vitro antioxidant and in vivo antidiabetic activity of the fraction were evaluated following standard protocols. Hyperglycemia was induced via intraperitoneal injection of 120mg/kg b. wt of alloxan monohydrate. Male Wister rats weighing between 120.20±15.25g was randomly distributed into five groups consisting of six rats each and administered 200 and 400mg/kg b. wt of the fraction, 10mg/kg b. wt of glibenclamide, and 2ml/kg b. wt of normal saline respectively. The quantitative phytochemical screening revealed the presence of phytochemicals such as phenols, flavonoids, tannins, saponins and alkaloids. The fraction exhibited antioxidant activity in a concentrationdependent manner with percentage inhibition against DPPH radicals compared with ascorbic acid. At 400 mg/kg b. wt the fraction reduced the blood glucose concentration of rats compared with the glibenclamide treated group after 21st day. The results of this study showed that chloroform fraction of Helicteres isora possess significant antioxidant and anti-diabetic activities.