Wound infections are considered significant health care problems, leading to high morbidity and mortality globally. Because wound infections are becoming a threat due to the acquisition of antimicrobial resistance by liable pathogens. The current study aimed to analyze the effect of iron oxide nanoparticles (IONP) such pathogens to overcome the issue. IONP is chemically synthesized from ferric chloride and ferrous sulphate as raw materials and co-precipitated to ferrous oxide. Production of IONP confirmed by UV-VIS spectrophotometry at 370nm and stability determined by zeta potential. Obtained dark brown color, fine powder of IONP appeared as flakes like thin discs, with a size range of 20.41 - 36.86nm in diameter under FESEM analysis. Presence of strong Fe-O stretch documented by the FTIR analysis and confirmed the formation of IONP. Synthesized nanoparticles showed high antimicrobial activity against methicillin-resistant Staphylococcus aureus. MIC was identified as 31.25 µg/ml and MBC was determined as 62.5 µg/ml. Antioxidant activity of synthesized nanoparticles was identified by DPPH scavenging assay as 61.4 µg/ml. SWISS ADME and ADMETSAR studies revealed favorable pharmacological properties with non-toxicity, high blood-brain barrier (BBB) penetration, and easy absorption ability. Interaction of iron oxide nanoparticles against Staphylococcus aureus surface protein 3TIP and 3TIQ showed a high binding affinity of -3.4 kcal/mol. Thus this study revealed the drug candidacy of iron oxide nanoparticles against methicillin-resistant Staphylococcus aureus.