Use of antifungal lactic bacteria and their bioactive metabolites is a good and sustainable biological way to fight fungi. In this study, 114 lactic strains isolated from raw milk of bovine, ovine, and caprine species were screened for their potential inhibitory activity against the fungus Penicillium glabrum. Among the isolated strains, 15 were found to exhibit antagonistic activity against the fungus in a direct in vitro confrontation test after incubation at 30°C for 48 hours. In the well diffusion method conducted using the supernatants of six isolates which showed strong inhibition, zones of inhibition around the wells, preventing the germination of Penicillium glabrum spores after incubation at 30°C for 48 hours, occurred in two of the supernatants. Inhibition was due to two lactic strains identified at the species level as Leuconostoc mesenteroides by 16S rRNA sequencing. These strains show a promising potential use in food bio-preservation