Tephrosia barberi (Fabaceae) J.R. Drumm is a perennial herb that has been used for abdominal pain, fever, peptic ulcer, vatham related diseases, liver disease, and skin disorders. The plan of study is to investigate the antioxidant and anti-inflammatory effects of Tephrosia barberi whole plant ethanol extract in lipopolysaccharide- induced RAW 264.7 murine Cells without causing cytotoxicity as well as the effects of carrageenan caused paw edema model in wistar rats. At 10 to 100 μg/mL concentrations ethanolic extract of Tephrosia barberi produced dose-reliant radical scavenging property by ABTS and DPPH assay methods. Mean while, inhibitory concentration of (IC50) ethanolic extract of Tephrosia barberi showed 29.80μg/mL and 31.83μg/mL by DPPH and ABTS methods, respectively. Pretreatment of RAW 264.7 macropage cells with ethanolic extract of Tephrosia barberi produced COX-2 inhibition and LOX inhibition at 48.69μg/mL and 43.50μg/mL (IC50) respectively in anti- inflammatory activity. Whereas