Withania coagulans Dunal and Stevia rebaudiana are useful plants with a significant role in Ayurvedic medicine. The presence of biomedical compounds as secondary metabolites confers antimicrobial, anti-inflammatory, anti-oxidant, and anti-cancer activities to medicinal plants. The total flavonoid and total phenolic content of Withania coagulans were determined using the aluminum chloride colorimetric and modified Folin- Ciocaltue methods respectively, while flavonoid content was measured by AlCl3 colorimetric method. Withania coagulans leaves obtained from Sargodha contained the greatest total phenolic content (47.33± 2.50 GAE/g DW). Mianwali leaf extract has highest flavonoid content QE/g DW (40.66 ±2.00 mg/g DW) and reducing power 32.662.5 Vit C equiv mg/g DW. Stevia rebaudiana leaves have total flavonoid content of 19.2±0.32, 22.9±0.18, and 13.79±0.17 mg Catechin/g in methanolic, ethanolic, and aqueous extracts, respectively. HPLC analysis of leaves of Stevia rebaudina revealed that rutin (0.14%), quercetin-3- D-glycoside (0.05%), and luteolin (0.03%) were most abundant phytochemicals in samples. Catechins were quantified at 280nm and included gallic acid (0.01%), epigallocatechin (0.72%), epicatechin (0.07%), gallocatechin (0.09%), and epicatechin gallate. To conclude phenolic content was highest in Sargodha leaves. Mianwali leaves extract had the greatest concentration of flavonoid.The total flavonoid content for the stevia rebaudiana plant was found in good concentration in all extracts.