This study focuses on documenting Austrocylindropuntia cylindrica (Lam.) Backeb populations and developing management strategies for them in Ermelo and Balfour, within the City of Mbombela Municipality, Mpumalanga Province, South Africa. Two new sites in Ermelo and Balfour, located in the Gert Sibande District Municipality, have been identified. Biological invasions pose significant threats to biodiversity, exacerbating climate change, water scarcity, fire risk, erosion, and flooding. Data were collected through site visits from 2021 to 2023, involving walking and recording GPS coordinates. The species is categorized under Category 1a of NEMBA regulations. This study aims to enhance understanding of this invasive species and propose management strategies. Limitations of the study include the need for further research on the species' behavior in other countries. It is recommended to monitor additional populations and increase awareness of biological invasions.