Background and Purpose: Medicinal herbs and traditional medicines have been used in our country since pre-Hispanic times and are significantly effective against a large variety of liver disorders. Bixaorellana is one of the herbal drugs used traditionally for fever, buccal tumors, antiseptic, antibacterial, and different types of hepatic ailments. To investigate the hepatoprotective activity of Bixaorellana L. (BO) shell extract against acute ethanol-induced hepatotoxicity in rats. Experimental approach:The characterization of BO shells extract was performed using standard biochemical analysis. 50% ethanolic extract of Bixaorellana (BOE 200 and 400 mg/kg body weight) was administered daily for 8 days in experimental animals for the assessment of hepatoprotective activity. To develop hepatotoxicity, animals were orally administered with alcohol (40%) 12 ml/kg at 2 h after the doses of BOE every day for eight consecutive days except the rats of normal group. The hepatoprotective activity was assessed using various biochemical parameters like aspartate aminotransferase, alanine aminotransferase, alkaline phosphatise, Bilirubin, albumin, Cholesterol and lactate dehydrogenase.