Background: Speckle tracking echocardiography (STE) is a novel, non-invasive technique for the evaluation of regional and global function of the left ventricle (LV). STE was suggested as an alternative method for evaluating LV torsion and deformation. We aimed to evaluate the LV torsion in diabetic patients using 2D STE. Methods: This single center, prospective study was carried out on 56 subjects recruited from Meoufia University Hospital. They were categorized into 2 groups; cases group (n=36) involved diabetic patients with preserved LV systolic function; EF ≥ 50% and control group (n=20) included age and sex matched individuals. All patients underwent general and local examination, conventional echocardiography and 2D STE. Results: The studied patients exhibited a significantly lower early diastolic myocardial wave velocity (E`) than the control group (P <0.05), and the ratio among early diastolic mitral inflow velocity and early diastolic myocardial velocity (E/e`) was significantly elevated in the trail patients contrast to in the control group (P <0.001). The control group exhibited a significantly higher apical rotation than the studied cases (P <0.001). Although the basal rotation of the patients under investigation was greater than that of the control group, it did not attain a statistically significant value (P >0.05). In summary, the torsion was significantly lower in the patients under study than in the control group (P <0.001). Conclusions: LV torsion by 2D STE was ignificantly lower in diabetic cases in contrast to controls so it should be measured in diabetic patients to detect early changes that correlate with LV dysfunction that can allow early detection and proper management.