An investigation into the lichen population was carried out in the southern Indian state of Tamil Nadu's Sanjeevani Hills. The study determined that the most prevalent families were Physciaceae and Parmeliaceae, focusing on four distinct locations in the hills. About a quarter of the total number of lichens were found at an elevation of 1300 m MSL on the 18th hairpin loop. This specific elevation above sea level was preferred by fructicose lichens, like Ramalina sp. Substrate specificity analysis revealed that 80% of the lichens were corticolous, 10% were saxicolous, and 5% were muscicolous. The present study is a preliminary investigation and a future reference for other researchers.