Background and Objective: Refractory Immune thrombocytopenic purpura is uncommon immunological disorders that is linked to various chronic medical conditions and various viral infections as HCV, HIV and CMV. The study aimed to evaluate the prevalence of CMV infection among patients with refractory ITP. Patients and Method: After approval of ethics committee, a total of 30 patients with refractory ITP were evaluated for clinical history, general and regional clinical examination. Patients were evaluated for CBC, serological markers of CMV (IGM and IgG) and bone marrow aspiration cytology. Results: The mean age of the studied patients was 34.8 years old with most of the studied patients were females (76.67%). Only one patient was found to have splenomegaly while 10 patients have history of splenectomy. Most of the studied patients didn’t have any chronic medical illness while 7 patients were diabetic and 4 patients have hypertension. None of the studied patients were positive for serological markers of HCV, HBV nor HIV. All of the studied patients with refractory ITP were found to have positive CMV IgG while only 1 patient had positive CMV IgM. Conclusion: CMV infection is highly prevalent among patients with refractory ITP. IgM for CMV is not recommended for screening of CMV infection among those patients.