In dentistry, prescribing antibiotics has become a routine aspect in recent years. Hence for limiting the antibiotic resistance pandemic, dentists must have adequate knowledge on antibiotic prescription guidelines. Aim and objective: The present survey targets to determine and analyze the level of knowledge and attitude of Antibiotics prescription guidelines among dental surgeons. Material and methods: 100 dental professionals with an age range of 23-40 years were included in the study. The questionnaire included 15 closed-ended, multiple choice questions. The first four questions include; general information such as gender, age, association with educational institutions, and years of clinical experience. The next eleven questions depict the attitude and awareness of Antibiotics prescription guidelines in dentistry. Results: The study highlights the need for better education on antibiotics prescription guidelines to the dentist and dental students. Conclusion: Majority of the participants have knowledge about antibiotic prescription guidelines, but to varying extent. The existing knowledge and approach should be improved. Continuing Education programs should be conducted to update them regularly about the newer drugs, updates on guidelines, and recent studies on the newer drugs might be of great help to the dental practitioners in limiting the antibiotic resistance pandemic.