Bacterial infections in hospitalized patients with liver cirrhosis in Egypt increased during last decades. We target using safe probiotics treatments to overcome multidrug resistant bacterial infection. Out of twenty patients recruited, MDR bacteria were isolated from five patients. The results indicated that both the total viable bacterial counts and resistance of MDR bacteria to different antibiotics reduced significantly after Lactobacillus acidophilus LB treatment. 16S rRNA results indicated that the two strains were E. coli ABA8 and K. pneumoniae DSM-30104. This research concluded the importance of using probiotics to avoid bacterial secondary infection in patients with chronic hepatic disease