We investigated the bacterial community in the leafhopper Matsumuratettixhiroglyphicus, a vector of sugarcane white leaf phytoplasma.Bacterial diversity was analyzed using V3-V4 16S rRNA metagenomic sequencing. A taxonomic analysis of the sequencing data revealed 43 known bacterial genera belonging to eight phyla, 16 classes, 30 orders, and 39 families. Bacteroidetes and Proteobacteria were the most abundant phyla in all samples, followed by Tenericutes, whereas phyla with relatively low abundances included Actinobacteria, Cyanobacteria, and Firmicutes. The Most abundant genus was primary symbiontSulcia, followed by unclassified bacteria from family Tremblayaceae.Another abundant genus was andPhytoplasma, which was detected exclusively in female leafhoppers. The remainder of the identified genera, including Acinetobacter, Bacillus, Agrobacterium, Propionibacterium, Pseudomonas,Sphingomonas,and Corynebacterium, were present relatively low abundances. To the best of our knowledge, this is the first report on the composition of the bacterial community in the leafhopper vector of sugarcane white leaf disease. The results may pave the way for the development of alternative strategies for the management of this insect.