As a result of harmful stimuli, the body's plasma and immune cells are drained from the rest of the body and directed toward the injured tissue. To encourage the regeneration of damaged tissue, a signaling cascade involving growth factors and cytokines propagates and matures in the inflammatory site. This cascade involves blood vessels and immune markers in the injured tissue. Objective: To evaluate the anti-inflammatory, wound healing and antioxidant properties of Phloretin in LPS stimulated L929 Fibroblast cell. Methods: L929 Fibroblast cells were used to study the wound healing efficiency of Phloretin. Wound healing efficiency of Phloretin was investigated by scratch assay. Phloretin was studied to see its ability to reduce nitric oxide production by Griess Assay, tumor necrosis factor – α expression in L929 Fibroblast were analyzed by ELISA. Phloretin’s effect on ECM Components analysis was done to check the content of Hydroxyproline and Hexosamine. Results: Phloretin reduces NO generation in LPS-exposed fibroblasts. Phloretin inhibited LPS-activated L929 fibroblast TNF-α production. LPS-treated phloretin had pronounced expression of hydroxyproline, indicating accelerated cell proliferation, collagen formation, and fast healing. The stability of hexosamine was also stabilized in comparison with the control.The recolonization and migration of cells was also augmented by scratch and chemotaxis assay upon treatment with Phloretin. Conclusion: In other words, this study suggests that reducing nitric oxide and unsettling the inflammation-causes eventful wound healing which is otherwise prolonged due to disproportionate chemokine and cytokine expression in pathological circumstances.