Background: interstitial cystitis/ Bladder pain syndrome (IC/BPS) is a chronic condition accompanied by lower urinary tract symptoms without evidence of urinary tract infection (UTI) last for at least 6 weeks. This condition affects women approximately five times more than men. The pathogenesis of the disease remains unclear Recently, IC/BPS has been classified into diffuse type and Ulcerative type with Honor’s ulcer is no longer considered to be a part of BPS/IC. . Up till now, there is no specific biomarker for detection of IC/BPS. Aim: To evaluate the level of nerve growth factor (NGF) in the blood and urine in females with non- ulcerative interstitial cystitis compared to healthy individual females. Subjects and methods: This study included 92 female participants: 46 females with non-ulcerative interstitial cystitis and 25 age- matched healthy individual females were included as a control group, and 21 samples was taken from patients diagnosed to have overactive bladder as another group. All patients underwent thorough history and clinical examination. Urine analysis and urine cultures, abdominal and pelvic grey scale ultrasound were carried out. Cystoscopy was conducted for all patients. Disease severity was assessed using 3-day voiding diaries, the Likert visual analog scale (VAS) for pain, and the Interstitial Cystitis Symptom Index (ICSI) and the Interstitial Cystitis Problem Index (ICPI) of the O'Leary-Sant questionnaire. The level of serum and urine NGF was determined by ELISA technique. The possible co relation between NGF and patients’ demographics, VAS, ICSI and ICPI was investigated.