This study employs a Quality by Design (QbD) approach to isolate boswellic acid from Boswellia serrata oleo gum resin. Solubility studies using ten different solvents, analyzed via thin layer chromatography (TLC), indicated that all solvents except water were effective for extraction. Among various extraction methods evaluated, maceration proved to be the most effective, though it required multiple extractions with fresh solvent to achieve complete extraction of boswellic acids. The extracted material was then concentrated, leading to significant evaporation of volatile oils. To isolate total acids, the resin was treated with alkali to convert the acids into their salt forms, followed by precipitation using a mineral acid, resulting in a white precipitate. The efficiency of KOH and NaOH as basifying agents was compared, with 2% KOH yielding the highest amount of isolated acid. This allowed for the successful isolation of acetyl keto β-boswellic acid (AKBA) and a mixture of α and β boswellic acids.