The research aimed to identify shrimp shell detritus and extract chitosan using an enzyme. We analyzed the molecular weight, liquid volume, protein concentration, and ash content of chitosan. Chitosan production was at 17.16 ± 0.21%, with an expected water content of 7.32 ± 0.05%. The demineralization process resulted in an ash concentration (0.1114 ± 0.002%) significantly lower than the 1% limit. Deproteinization revealed a protein concentration of 1.12 ± 0.02%, with approximately 97.02 ± 0.05% of the chemical being soluble. The average molecular mass was 109.27 ± 0.34 KDa. Fourier transform infrared spectroscopy (FTIR) and XRD diffraction pattern analysis helped understand the chemical makeup, functional groups, and crystal structure of chitosan. Its antioxidant capabilities were tested using the DPPH technique, showing a 72% scavenging capacity and potent antioxidant activity similar to BHA at a concentration of 1%. These studies demonstrate the multiple potential uses of chitosan.