Seagrass possess various pharmacological active compounds which have been utilized for the biosynthesis of silver nanoparticles and act as biocompatible, stable and reliable alternative instead of the current chemical and physical approaches. Enhalus acoroides possesses various pharmacological properties and the present study was aimed at assessing the chemical characteristics and anticancer effects of synthesised silver nanoparticles (AgNPs) with the extracts of E. acoroides against HeLa cell lines. The nanoparticle was synthesised from the aqueous extract and characterisation of nanoparticles were carried out using UV-Vis, FTIR, SEM, TEM and EDX analysis. Further these nanoparticles were subjected to cytotoxicity study using HeLa cell line. The UV-Vis spectrum at 200- 1100 nm and FTIR analysis revealed various functional groups which corresponds to numerous phytochemicals like alkaloid, flavonoids and polyphenols. The crystalline structure of the nanoparticle synthesised was confirmed using XRD, which was exhibited by silver nanoparticles by EDX analysis. The spherical shape and average size of the synthesised nanoparticles was visualised. The morphological characteristics was confirmed using SEM and TEM. The IC50 value was found to be 72.89 μg mL-1 demonstrating the potency of silver nanoparticles against the HeLa cell line. The cytotoxicity assay using MTT revealed that the synthesised nanoparticle exhibited a dose-and time-dependent cytotoxicity against HeLa cell lines. Thus, the study concluded that E. acoroides silver nanoparticle is an effective strategy for cervical cancer treatment.