ISSN : 2663-2187

Chemical Profile, Radical Scavenging, Reducing Power, and Anti-Lipid Peroxidation Activities of Lavandula stoechas Methanol Extract and Its Fractions

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Habiba Djenidi , Hind Amira, Fatima Benchikh, Walid Mamache, Hassiba Benabdallah, Asma Ouaret, Islam Amira, Aya Amira, Mohamed Abdallah Torki, and Smain Amira
» doi: 10.48047/AFJBS.6.15.2024.10626-10634

Abstract

Lavender has long been believed to offer various therapeutic and curative benefits, such as promoting relaxation and addressing conditions like parasitic infections, burns, insect bites, and muscle spasms. In this study, the aerial parts of the plant, collected during its flowering phase, were extracted using 85% methanol over a period of three days. The resulting suspension was then filtered through a Büchner funnel, and the methanol was evaporated under reduced pressure using a rotary evaporator, yielding a crude methanol extract (ME). This extract was subsequently partitioned with hexane, chloroform, and ethyl acetate, followed by evaporation to dryness under reduced pressure, to obtain hexane (HE), chloroform (CHE), ethyl acetate (EAE), and remaining aqueous (AqE) extracts. The total phenolic and flavonoid content of each extract was then assessed. The antioxidant properties of these extracts were evaluated through three in vitro assays: ABTS radical scavenging activity, reducing power, and hydrogen peroxide scavenging. Results showed that all extracts were rich in phenolic compounds, with values ranging from 55.85 to 104 mg gallic acid equivalents per gram of dry extract. Flavonoid levels varied from 12.36 to 32.65 mg quercetin equivalents per gram of dry extract. Notably, the ethyl acetate extract demonstrated the highest radical scavenging activity in the ABTS assay (IC50 = 1.45 μg/mL), along with strong reducing power (EC50 = 23 μg/mL) and hydrogen peroxide scavenging ability (IC50 = 95 μg/mL). These findings suggest that lavender is a promising source of natural antioxidants

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