Background:Macrocytosis is a common finding encountered in automated coulters and evaluation of clinical, biochemical and haematological parameters in macrocytic anemias will provide a clue to diagnosis. This study was done to evaluate the clinical and laboratory parameters and distribution of macrocytic anemia in our hospital and we also studied the mean difference between the gender and laboratory parameters of macrocytic anemia. Methods: A cross sectional study was conducted in Saveetha medical college a tertiary care hospital, Chennai. 50 patients presenting with macrocytosis were taken in to study. A detailed clinical history and physical examination was done in all cases. Complete blood count (CBC), peripheral blood examination and reticulocyte distribution width – coefficient of variation (RDW-CV) was done in all cases. Results: Majority of study participants (48%) were in age group of 41-60 years with male: female ratio 2.1:1, with 70% vegetarian diet. The most common presentation of the patient was easy fatigability (56%) and commonest sign was pallor (86%). The laboratory findings suggestive of macrocytic anemia were the mean hemoglobin levels 9.04+_1.85mg/dl, mean MCV 104.07+_3.72fl, mean RDW-CV 15.61+_2.61%, with peripheral smear suggestive of megaloblastic anemia in 64 % and 28% had bicytopenia, while 8% had pancytopenia. The mean difference between the gender and RBC count, MCV, MCHC, WBC count were statistically significant with p value<0.05.