INTRODUCTION: Platelet-rich fibrin (PRF), a second-generation platelet concentrate containing platelets and leukocytes in a fibrin membrane, has shown promise as a therapeutic tool for tissue repair. However, there is still ongoing debate regarding the superiority of different types of platelet concentrates, in promoting periodontal regeneration. AIM: The aim of this study is to compare and assess the differences in the Structure of L-PRF and A-PRF+ with and without the addition of antibiotics using Scanning Electron Microscopy analysis. MATERIAL & METHODS: Blood was collected from 20 subjects and 0.5 ml of antibiotics were added to respective tubes and centrifuged as per protocol to obtain L-PRF and A-PRF+ following which they were made into respective membranes. The samples were further processed for SEM analysis. RESULTS: A-PRF + membranes had thicker density fibrin network when compared to L-PRF membranes. The distribution of leukocytes and platelets were found to be more homologous in A-PRF+ membranes when compared to L-PRF membranes. With addition of antibiotics more thicker fibrin networks were obtained. CONCLUSION: Within the limitations of the study,there were structural differences in PRF membranes .Further studies are essential for more conclusive results.