Biochemically injected calcium carbonate (CaCO3) precipitation (BICP) is emerging as an innovative, sustainable method for boosting the strength and longevity of concrete structures, offering a greener alternative to conventional repair practices. While urea-dependent MICP is prevalent due to its efficient CaCO3 precipitation rates, the byproduct ammonia (NH3) poses risks to cementitious structures and environmental health. In response, developments have been made in self-healing concrete systems utilizing Non-Ureolytic Bacteria (NUB), offering a safer and more sustainable solution. This study explores the use of standardized cells from a known NUB bacterium, Bacillus subtilis (B. subtilis), to enhance the Compressive Strength (CS) and Water Absorption Resistance (WAR) of concrete, comparing bacterial concrete to conventional concrete. B. subtilis, a Gram-positive bacterium, was utilized for its ability to precipitate calcite on media infused with a calcium source. Concrete cubes were cast using four different bacterial doses (30, 50, 70 and 100 percentage replaced with water), alongside control specimens without bacteria. The results indicated a notable improvement in CS and WAR with bacterial addition, particularly at a concentration of 70 percentage replaced with water, where increases of 26% and 3.5% were observed after 28-days and 56-days of curing, respectively. The study concludes that bacterial calcite significantly enhances the strength and WAR resistance of concrete compared to conventional methods.