Background: Endo-perio lesions, characterized by the simultaneous presence of endodontic and periodontal diseases, present a complex clinical challenge. The traditional treatment involves a combination of endodontic therapy and periodontal intervention. Recent advancements suggest that adjunctive use of diode lasers may enhance treatment outcomes by reducing microbial load and promoting tissue healing. This study aims to compare the efficacy of a standard treatment protocol with one extended by using a diode laser for managing endo-perio lesions. Materials and Methods: A total of 60 patients with diagnosed endo-perio lesions were randomly assigned to two groups. Group A (n=30) received the standard treatment protocol, including root canal therapy and scaling and root planing (SRP). Group B (n=30) received the same standard treatment, augmented with diode laser therapy applied at 810 nm wavelength for decontamination. Clinical parameters, including probing depth (PD), clinical attachment level (CAL), and bleeding on probing (BOP), were recorded at baseline and six months post-treatment. Microbiological analysis was performed to assess bacterial reduction.