Oxadiazoles belong to the group of heterocyclic compounds which contains one oxygen and two nitrogen atoms, forming a five-membered heterocyclic ring. The current research was based on the design, synthesis and pharmacological evaluation of novel 2-[3-(4-Hydroxy methyl benzoyl) propionic acid-5-(Substituted phenyl)-1,3,4-Oxadiazole Derivatives.The novel derivatives of oxadiazole were synthesized by utilizing specific conditions and process and evaluated their physical properties including melting point, RF value, FTIR, NMR, Mass, and Docking studies.The animals were obtained from animal house, Department of Pharmacy, MJPRU Bareilly for in-vivo study.The anti-inflammatory activity of synthesized novel oxadiazole derivatives were tested by carrageenan-induced paw edema. In results, when compared anti-inflammatory activity among all compounds, D3 and D4 were found excellent moiety in terms of maximum % inhibition comparable to standard group. Novel oxadiazole derivatives synthesized successfully and exhibited significant anti-inflammatory activity at both the doses used. The response was noted as dose-dependent. Maximum inhibition was recorded in the dose of 200mg/kg of oxadiazole. In conclusion, novel oxadiazole derivatives might be much significant in reducing the inflammation and counter it. Maximum percentage inhibition was obtained in D3 and D4, respectively.It suggests to perform the structure elucidation of synthesized derivatives and develop after structural modification (SAR) in desired dosage form to avail the highest potency and efficacy (intrinsic activity). It also needed to further researchers to determine its mode of action for anti-inflammatory response.