Introduction: Pseudomonas aeruginosa is a gram negative bacterium which causes opportunistic infections. It has major role in healthcare associated infections (HAI) like ventilator associated pneumonia(VAP),burns, surgical site infections. Biofilm formation among the isolates has led to poor prognosis which aggravates the infections and increase in antibiotic resistance causing increase in morbidity and mortality. Aims and objectives:1.Isolation and identification of Pseudomonas aeruginosa2.To detect biofilm formation among Pseudomonas aeruginosa isolates