The aim of this research work was to prepare a Nanofibrous scaffolds of Nelumbo Nucifera Rhizomes for tissue regeneration. Electrospun Nanofibers of Nelumbo Nucifera Rhizomes was prepared and evaluated for Field emission scanning electron microscopy, Fourier transform-infrared spectroscopy analysis, Zeta potential, X-ray diffraction, Differential Scanning Colorimetry, Swelling index, In-vitro drug release study, Antibacterial activity, In-vitro anti-inflammatory study and Accelerated stability study. Here excision wound model was used to evaluate the tissue regeneration activity of formulation in- vivo. FESEM reveals the formation of smooth nanofibers with a uniform diameter. The degree of swelling of Nelumbo Nucifera Rhizomes Nanofiber in simulated wound fluid (pH 7.4) was found at 390.12% up to 12 hr. Within 24 hr 96% drug was released from Nelumbo Nucifera Rhizomes Nanofiber Scaffolds. The antibacterial action was enhanced by the Nelumbo Nucifera Rhizomes nanofibers by showing larger zone of inhibition. Nelumbo Nucifera Rhizomes nanofibers exhibited around 64.16% of anti‑inflammatory activity at 200 μg/ml. Tissue Regeneration Activity of nanofibrous scaffolds of Nelumbo Nucifera in Wistar Rats demonstrates considerable potential for advancing wound healing in rats. It also revealed that prepared scaffolds increase the platelets count that is necessary for acceleration of Tissue Regeneration.