This study aimed to fabricate and develop gastroretentive mucoadhesive microsphere of gabapentin followed by its characterization and evaluations. The microsphres were prepared using ingredients including Na-alginate, CaCl₂, and HPMC or Chitosan. Fourier Transform Infrared Spectroscopy (FTIR) was used to analyze chemical interactions. The formed microspheres were filtered, washed, and dried at room temperature or in an oven. Particle size and morphology were determined using optical microscopy and SEM. Drug loading and encapsulation efficiency were assessed using UV-Visible spectrophotometry. Swelling index, mucoadhesion, and in vitro drug release studies were conducted to evaluate the microspheres' properties and effectiveness. Encapsulation efficiency and drug loading capacities were high, particularly for GAB-1, GAB-2, and GAB-6, which exhibited efficiencies above 98%. SEM analysis revealed uniformly spherical microspheres with rough and porous surfaces, enhancing mucoadhesion and drug loading capacity. Particle size analysis confirmed that formulations like GAB-2, GAB-4, and GAB-6, with spherical shapes and moderate sizes, are optimal for consistent drug release and gastric retention. In vitro drug release studies indicated that GAB-1, GAB-2, and GAB-3 had the most extensive release profiles, while GAB-4, GAB-5, and GAB-6 were suited for controlled release applications. The zero-order kinetic model showed the highest R-squared values across all formulations, indicating a consistent drug release rate. Specifically, GAB-1 (0.9887) and GAB-4 (0.9876) demonstrated the most predictable release profiles. The Korsmeyer-Peppas model, with release exponent (n) values between 0.6 and 0.68, suggested a complex release mechanism involving both diffusion and erosion. The findings demonstrated successful fabrication of gastroretentive mucoadhesiuve microspheres of gabapentin.