The current study focuses on the development and in vitro characterization of fluconazole encapsulated niosomes to improve drug delivery and therapeutic efficacy. Niosomes, which are non-ionic surfactant vesicles, offer significant advantages in enhancing the bioavailability of drugs with poor water solubility. Fluconazole, an antifungal agent with limited water solubility, was encapsulated in niosomes using the thin-film hydration method. The niosomes were characterized through particle size analysis, zeta potential measurement, encapsulation efficiency, in vitro drug release, and stability studies. The results revealed that the niosomes had a uniform particle size distribution, high encapsulation efficiency, and sustained drug release profile. Stability studies indicated that the niosomes remained stable under various storage conditions. This study demonstrates that fluconazole encapsulated niosomes could potentially enhance the bioavailability and therapeutic performance of fluconazole, paving the way for further in vivo studies and clinical applications.