Background: Gynecomastia involves non-cancerous enlargement of the male breast due to glandular tissue proliferation, affecting 40 to 55% of healthy men. Recent technological advances, particularly compact and dependable diode lasers operating at wavelengths like 810, 915, 940, and 980 nm, have demonstrated promising outcomes as reliable, safe, and effective alternatives to conventional liposuction. Our aim was to assess the therapeutic efficacy of the 980 nm diode interstitial laser in treating gynecomastia. Methods: This study is a prospective, cross-sectional case series involving 36 patients undergoing laser-assisted liposuction for gynecomastia treatment. The Rohrich system was used to assess the severity of gynecomastia, with the senior author determining the required amount of breast gland to be removed for each patient. Following surgery, patients are assessed in the clinic on the fifth day to evaluate bruising or ecchymosis, and a chest binder is applied. Their next follow-up appointment is scheduled one month later. Patients' photographs are taken on the fifth day post-surgery, and again at one month and six months post-surgery. Postoperative swelling, pain, skin laxity, amount of aspirated blood, and any complications are closely monitored during this period. Results: Right after surgery, all patients experienced swelling and minor bruising, which generally subsided within 10-14 days following the procedure. Overall, the surgical outcomes were deemed satisfactory in every case. Among the 36 patients, 28 (77.7%) were satisfied with their cosmetic results, while eight (22.3%) expressed dissatisfaction. One patient needed additional liposuction and glandular treatment due to under-correction, and another required corrective measures for skin irregularities. Significant skin tightening was noted in several instances. There were no reports of hematoma, seroma, infections, or other early postoperative complications. Conclusions: Diode 980nm interstitial laser could effectively treat gynecomastia without postoperative complications.