Sericulture is an important agro-based industry in India that produces all four types of commercially exploited silk, viz Mulberry, Eri, Tasar & Muga. Among these silks Mulberry silk accounts more than 80% total silk production in India. As mulberry leaf production alone covers more than 60% of the total production cost, mulberry leaf production plays a crucial role in the sustainability of Sericulture in a region. Keeping this in view research organization in India have developed a number of mulberry varieties suitable to different agro-climatic condition of India. G4 is one such mulberry variety that has been developed by Central Sericultural Research &Training Institute, Mysore with high productivity. Since the high leaf yield production potential mulberry varieties requires high input to produce quality leaf in higher quantities, large quantities of organic and chemical fertilizers have been recommended for their cultivation. For G4 mulberry verity the current recommended fertilizer dose is NPK of 350:140:140. However continuous application of high dosage of chemical fertilizers affect not only the soil structure but also the micro flora and funa of the soil. In order to minimize the negative impact of chemical fertilizers efforts have been made to replace chemical fertilizers partly of completely with organic fertilizers such as FYM, Cow dung, Poultry manure and other farm residuals. In southern part of India large numbers of poultry farms are operating to carter to the need of the meat industry in the region. From these poultry farms huge quantities of poultry manures are been produced daily. In order to utilize these manures effectively and efficiently for the production of mulberry leaf the present experiment was undertaken using G4 mulberry gardens. In this study the effect of application of different compositions of chemical, organic and poultry manures where evaluated.