Objective: To determine the lipid lowering and atherogenic index lowering potential of Lycopene in high sucrose diet fed male Wistar albino rats. Study design: Experimental study Study setting: Department of Biochemistry, Liaquat University of Medical and Health Sciences. Duration of study: 6 months from 1-10-2022 to 30-3-2023 after approval from the Ethical Research Committee, LUMHS. Methodology: 60 male Wistar albino rats were randomly divided into 4 groups (15 rats in each group); Group A (negative control), Group B (positive control), Group C (SRD + lycopene 20mg/kg) and Group D (SRD + lycopene 40 mg/kg). After 4 weeks of experimental period serum triglycerides and High density lipoprotein cholesterol levels were analyzed. Data was analyzed on SPSS 23.0 version at 95% confidence interval (p ≤ 0.05). Results: Serum HDLc of group A, B, C and D were found as 46.60±5.8 (SEM 1.5) mg/dl, 25.93±4.23(SEM 1.09) mg/dl, 36.13±7.2 (SEM 1.87) and 43.07±1.08 (SEM 1.08) mg/dl (P=0.0001) and Serum Triglycerides of group A, B, C and D were found as 70.3±14.8 (SEM 3.8) mg/dl, 100.8±19.2 (SEM 3.2) mg/dl, 92.5±13.2 (SEM 3.4) and 79.6±14.7 (SEM 3.8) mg/dl (P=0.0002). Conclusion: It is concluded that lycopene was found effective in lowering serum triglycerides and elevating HDLc.