The intent of this study was to analyze the effect of six-week aerobic training and four treatments (Calcium, Iron, Combo and Placebo supplementation) on the resting biochemical parameter i.e. calcium level among selected school level athletes. To conduct the investigation, 60 male athletes from state sports hostel, Una, Himachal Pradesh ranging from 12 to 19 years age group were selected as subjects on the basis of random sampling. They were further divided into four groups with15 subjects in each group and the groups were named as Calcium, Iron, Combo (Calcium-Iron) and Placebo treatment groups. The four groups undertook a planned aerobic training of six weeks duration and each group was regularly administered one of the Calcium, Iron, Combo (Calcium-Iron) and Placebo supplementations respectively. It was hypothesized that there would be no significant effect of aerobic training and four treatments (Calcium, Iron, Combo and Placebo supplementation) onthe resting biochemical parameter calcium level among selected school level athletes. To cater to the need of the study resting blood samples were obtained from the subjects in the morning i.e. on day-0, day-21 and day-42 and these blood samples were tested in the biochemistry laboratory at IGMC, Shimla. The data obtained was analyzed with the application of one-way repeated measures ANOVA and post hoc test. The result of study exhibited that the four treatments along with aerobic training had a significant effect on the resting calcium level of athletes in the form of increased levels of calcium from one point of time to another with minor exceptions.