Aim: Evaluating the effectiveness of laser assisted and ultrasonic irrigant (UI) activation on the depth of penetration of AH PLUS sealer Methods: Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines were followed during the review process. Databases were searched from January 2000 to December 2023 for studies evaluating the effectiveness of laser assisted and ultrasonic irrigant (UI) activation on the depth of penetration of AH PLUS sealer. Quality assessment was evaluated using Cochrane risk of bias (ROB) -2 tool for randomized controlled trials (RCT) through its domains using Review manager (RevMan) software version 5.3. With a random effect model (p<0.05), the standardized mean difference (SMD) was employed as the summary statistic measure.