Ornamental fish trade is based on their brilliant colors and patterns, which frequently determine the fish's market worth. The aim of the study was to evaluate the effect of dietary marine microalgae Chlorella salina on the growth, digestibility, hematological, antioxidant enzyme activities and coloration of Tricogasterlalius (Flame red gourami). The experiment trial was conducted for 60 days by feeding different formulations of C. salina (100% algal feed (group I), algal feed mixed basal diet in the ratio of 1:1 (group II)) and a control diet with no microalgae added for comparison. From the results, the dietary inclusion of C. salina rich feed showed its effectiveness in enhanced growth performance, digestive enzyme, hematological and antioxidant activities in T. lalius when compared to the control. Color measurement lightness L* (70.19±0.12), Redness a* (4.51±0.23) and Yellowness b* (14.7±0.22) values were maximum found in T. laliusfed with C. salina rich diet after 60 days of feeding trials. It was found that increasing the amount of C. salina in the fish diet increases carotenoid levels in T. lalius skin. This study suggests that C. salina rich diet might be used as a necessary feed supplement as well as a coloring agent in the culture of ornamental fish T. lailus.