Plants are tremendous sources of different macro and micronutrients that are involved in diverse physiological functions in human body. Quantification of different macro- and micronutrients in traditionally famous medicinal plants is very essential from nutritional and therapeutic perspective. In the present study, micro- and macronutrient contents were determined in 10 medicinal plants collected locally in upper Assam, India to score the nutrients efficacy of the plants. Following standard estimation protocols, moisture content, ash percentage, micronutrients (Fe and Cu) and macronutrients (Na, K, Ca, Mg and P) were determined in the leaves of selected plants in dry weight basis. The results of the current study have documented the maximum Cu concentration (0.142 mg g−1) in the leaves of Sessile Joyweed and the available P content (1.62 mg g−1) in Fenugreek leaves. However, Cu (0.007 mg g−1) and P (0.107 mg g−1) concentrations were found to be lowest in Mint and White Goosefoot. Fe concentration was maximum (0.331 mg g−1) in Skunkvine, while the lowest but nearly similar results of this element were found in Pungent Leaf (0.109 mg g−1), Sessile Joyweed (0.109 mg g−1) and Green Amaranth (0.112 mg g−1) respectively. In this study, the highest moisture content (9.89 %) was recorded in the leaves of Amaranth, but ash percentage (7.84 %) and Ca (3.22 mg g−1) content were found maximum in Green Amaranth. Similarly, K (9.29 mg g−1) and Na (5.02 mg g−1) were found highest in Spinach and Sessile Joyweed. The results of K (5.54 mg g−1) and Na (2.31 mg g−1) were lowest in Pungent Leaf. In contrast, the lowest Ca and Mg were recorded in Mint (1.57 mg g−1) and White Goosefoot (0.51 mg g−1). However, Mg content was highest (2.09 mg g−1) in the leaves of Spinach. The findings of the current study have revealed that elemental quantification in different medicinal plants is very relevant in terms of therapeutic use and nutritional intake.