Polyphenolic chemicals represent a significant category of plant secondary metabolites that are prominent for their extensive variety of therapeutic activities. These chemicals are typically found in plants in trace amounts and notably, exhibit a diverse range of therapeutic properties for human health. Medicinal plants are utilized in the field of pharmaceuticals owing to their cost-effectiveness, limited occurrence of unwanted effects, and significant contribution to traditional therapeutic practices. Owing to this, there is a widespread global exploitation of these plants, resulting in the inclusion of numerous medical plant species on the endangered list. Plant tissue culture manifested itself as a promising alternative for obtaining these chemicals, and their production can be enhanced through the process of elicitation. Alpinia purpurata belonging to family Zingiberaceae is a medicinal plant with myriad therapeutic effects viz. antibacterial, antioxidant, vascular activity, acute hypotensive effects, cytotoxic, apoptotic properties and anti-cancer activity. The current study assessed the impact of signalling compounds, methyl Jasmonate (MJ) and salicylic acid (SA) as elicitors, on in vitro shoot cultures of A. purpurata. Maximum growth of the in vitro shoot cultures and flavonoid accumulation was observed on the 25th day, as determined by growth studies conducted by harvesting shoot cultures at regular intervals of 5 days during a 40-day period. The shoot cultures were treated with elicitors for 24-96 h, on 24th day followed by post-elicitation analysis for methanol extraction with dried plant material. Each of the characteristics evaluated was significantly influenced by the type of elicitor and the duration of exposure. In comparison to the control, all treatments significantly increased flavonoid synthesis. The findings obtained from the reverse phase high performance liquid chromatography (RP-HPLC) analysis revealed that shoot cultures exposed to MJ at a concentration of 200μM for 48h displayed the highest level of all the three flavonoids Myricetin (743.16±5.99 µg/g DW), Quercetin (646.15±2.15 µg/g DW), and Kaempferol (180.25±2.12 µg/g DW). When compared to the controls a threefold increase was observed for all the three flavonoids. SA mediated elicitation of shoots after 48 h at a concentration of 100 μM, exhibited a two-fold increase for all the threeflavonoid’s Myricetin (494.26±3.18 µg/g DW), Quercetin (431.12±3.25 µg/g DW), and Kaempferol (120.83±1.21 µg/g DW) compared to the control. Our findings indicated that MJ proved to be superior elicitor than SA for enhancing the synthesis of flavonoids in shoot cultures of A. purpurata.