Euphorbia helioscopia is a well-known species of medicine plant in the Euphorbiaceae family. In traditional medicine, euphorbia plants have been used for hundreds of years to treat warts, tumors, and cancer. The study's goal was to find out how harmful the water-based extract of Euphorbia helioscopia was to Swiss white mice. After giving the extract by mouth in doses of 2 g/kg and 5 g/kg, each patient was watched for four hours on their own. After that, they watched them nonstop for 24 hours and then at least once a day for the next 14 days. According to the study's findings, the liquid extract of Euphorbia helioscopia did not cause any harm or death in any of the treatment groups that were given it. On top of that, there were no changes between the treatment and control groups in body weight, food intake, organ weights, or biochemical markers. Since this is the case, giving Euphorbia helioscopia extract to mice by mouth is thought to be safe and not cause any harm.