Kayakarpam is a unique therapeutic division in the Siddha system of medicine that helps in rejuvenation, longevity, and spiritual well-being. The great saint Thirumoolar has explained the concept of Kayakarpam in his text Thirumanthiram. Kayakarpam can be correlated to antioxidants. And most human diseases are associated with inflammation. There is a dire demand for the development of novel, efficacious, and safe anti-inflammatory drugs preferably from natural sources. To explore the literature evidence scientifically, antioxidant and anti-inflammatory activities are carried out for Siddha polyherbal formulation Panchadeepakini Ilagam (PDI) using DPPH assay and Albumin Denaturation assay respectively. In Siddhavaithiya thirattu, PDI is indicated for the vatha disease, pithavayu, valli. The main ingredients of PDI are Sukku (Zingiber officinale), Milagu (Piper nigrum), Thippilli (Piper longum), Elam (Elettaria cardamomum), Seeragam (Cuminum cyminum). The result indicates that the polyherbal Siddha formulation PDI decreased oxidative stress via its antioxidant properties and was found to be an effective scavenger of DPPH and it also possesses significant Anti–inflammatory activity. Further elaboration of this study with In Vivo screening will be more helpful for the drug for clinical application. Thus, it is concluded that Panchadeepakini Ilagam has Anti-oxidant activity and Anti-inflammatory activity.