In India, Trigonella foenum-graecum, an evergreen tree, is commonly used by traditional practitioners for gastric ulcer healing. In order to determine whether Trigonella foenum- graecum seed aqueous extract (AE) has antiulcer properties, this investigation was conducted in a lab. “Using an in-vitro technique called the H+/K+- ATPase inhibitory activity method, the anti-ulcer actions of different dosages of the aqueous extract (100 mg, 500 mg, 1000 mg, and 1500 mg) were evaluated. When compared to the gold standard of Aluminum hydroxide and Magnesium hydroxide (500 mg), the extract showed a drastic reduction in acid neutralizing capacity (ANC).” The antiulcer properties of Trigonella foenum-graecum seed powder have been extensively studied, highlighting the efficacy of its active constituents. Stress, long-term use of anti-inflammatory medicines, and other unknown variables have all been linked to on various native plants and their derivatives to treat peptic ulcers. The onset of ulcers in people. The fundamental process is a mismatch between aggressive elements and the body's inherent defensive systems for keeping the mucosal lining intact. Traditional healers have long relied. The main objective of this study is to estimate the in-vitro antiulcer activity of Trigonella foenum-graecum seed aqueous extract (AE).