Fruits of Diospyros melanoxylon (Tembar) were collected from local reasion Mudakhed, Bhokar, Kinwat (Nanded) Golatgaon. Shadedried &Powdered Diospyros melanoxylon fruits (100 gm.) were extracted using a Soxhlet extractor sussesively & with hydroalcoholic solvent system in the ratio 1:1, temperature set at 60-70 oC. Hydroalcoholic extract of Diospyros melanoxylon fruits preliminary phytochemical analysis was done using standard methods. phytoconstituents were isolated i.e., gallic Acid [3,4,5-trihydroxybenzoic acid] from n- butanol hydroalcoholic extract of fruits of Diospyros melanoxylon (NHDM). Phytoconstituent isolation is an important concluding result of the study and cardioprotective activity of the two extracts may be attributed to these phytoconstituents. The hydroalcoholic extract of fruits of Diospyros melanoxylon was evaluated for immunomodulatory activity in laboratory animals namely anti-inflammatory activity using carrageenan induced paw edema model, immunomodulatory activity using carbon clarence test, immunomodulatory activity using E. coli induced abdominal sepsis (Determination of Host Resistance). The extract showed potent immunomodulatory activity. The multistep putative action of these plants may be attributed to the prominent phytoconstituents namely i.e., gallic Acid [3,4,5-trihydroxybenzoic acid] from n- butanol hydroalcoholic extract of fruits of Diospyros melanoxylon (NHDM).