To study the anti-ulcer properties of a controlled release preparation with aqueous extract from verbena hastate in rodent models. Methods: Microspheres were synthesized using a solvent evaporation technique, and their characteristics, including scanning electron microscopy images, stability, and in vitro performance, were systematically assessed and verbena hastate loaded microspheres were assessed in an in-vivo study using rats with induced gastric ulceration via ethanol/HCl. parameters examined included pH, Ulcer Index, Total acidity, and histopathological studies. Results: The findings revealed that Verbena hastata harbors key active components, notably flavonoids, attributing to its prowess in combating ulcers. In addition, the study observed that microspheres containing an aqueous extract displayed the highest activity, specifically at a dosage of 200 mg/kg. This was in comparison to the standard reference, Cimetidine, which exhibited activity at a dosage of 100 mg/kg. This finding suggests a potentially enhanced or superior effect associated with the microspheres loaded with the aqueous extract in the context of the study, Additionally, the study outcomes unveiled that the administration of aqueous extract microspheres at a dosage of 200 mg/kg led to a significant reduction in ulcer incidence. This was evidenced by a notable reduction in the ulcer score when compared to the normal control group in the experimental model. Conclusion: The results suggest that microspheres loaded with verbena hastata aqueous extracts demonstrated effective antiulcer activity and safety.