Hypochaeris and Centaurea are endemic plants belonging to the Asteraceae family, localized in the eastern Algeria. The objective of this study was the evaluation of the antibacterial and antifungal activity of their dichloromethane, AcOEt and n-butanol extracts using the method of diffusion by discs on five strains with Gram- and Gram + and the method of wells on three phytopathogenic fungal strains. The results revealed that the bacterial strains are very sensitive to the dichloromethane extract of H. Laevigata and C. Papposa blue with significant inhibition zones, with the fungal strains being sensitive to n-butanol of H. Laevigata and C. Papposa blue dichloromethane extracts. The richness of these two species in several secondary metabolite classes could give them an important therapeutic and medicinal value. The presented results could provide an overview of the antibacterial and antifungal potential of extracts from these plants.