The uses of plant and animal-derived products as medicines have long record to treat and manage different types of diseases. The combination of Camellia sinensis (C. Sinensis) and cow urine is never been investigated in regards to its sun protection factor (SPF), antioxidant and antibiofilm activities. The combined activity of both extracts showed potent photoprotective activity (SPF value33.75±0.25), and antioxidant activity with free radical inhibition was 73.94%. Natural products can avertible oxidative stress and act as antioxidants that are used as potent sunscreen. According to the Congo red assay, Escherichia coli, Klebsiella pneumoniae, and Listeria monocytogenes displayed robust biofilm formation capabilities, while Salmonella typhi, Pseudomonas aeruginosa, and Staphylococcus aureus exhibited moderate biofilm formation tendencies. Candida albicans, on the other hand, showed a weak tendency for biofilm production. The combined extract demonstrated significant antibiofilm effects, particularly potent against Listeria monocytogenes (96.28%), followed by Klebsiella pneumoniae (79.62%) and Escherichia coli (76.91%). It is concluded that combined formulation could be used as an alternative remedy against to biofilm formation to avoid the various adverse effects of synthetic drugs.