The current study's objective was to identify the antibacterial properties and phytochemical content of Trapa natans L. (Family: Trapaceae) Fruit peel and Leaves extract. The well recognized testing protocol described in the literature was used to determine the qualitative analytical evaluation of various phytochemical components and the quantitative analytical evaluation of total phenolics and flavonoids. By using the Folins Ciocalteau reagent and the aluminium chloride method, quantitative analysis of phenolics and flavonoids were conducted, respectively. S. mutans, S. bongori and Escherichia coli were subjected to an in vitro antimicrobial capability test utilising the well diffusion assay technique and standard ofloxacin and ciprofloxacin (10–30 micro g/ml). The millimetre (mm) diameter of the zone of inhibition was used to calculate the antibacterial activity. According to the phytochemical examination, tannins, glycosides, flavonoids, alkaloids, etc. were present. The hydroalcoholic extract of following plants: Trapa natans L fruit peel and leaves had antibacterial activity against all pathogens in a concentration-dependent manner, but it was less potent than conventional medicine. It has been determined that the presence of these phytochemicals is what causes the plant to exhibit antibacterial action. Future research may focus on isolating and modifying the phytochemicals that are responsible for these actions.