New approaches are needed to control tuberculosis (TB) worldwide. In particular, new tools for detection and novel biomarkers are needed to assess key pathogen and host components in the response to infection. Angiopoietin-like 4 (Angptl4) is a protein that belongs to the angiopoietin-like family. Angptl4 promotes the recruitment of immune cells and the production of inflammatory cytokines, helping to modulate inflammatory processes. It can be induced by pro- inflammatory signals, such as tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β) and lipopolysaccharides (LPS). Due to its multifaceted role in metabolic disorders, angiogenesis and inflammation, Angptl4 has attracted attention as a potential therapeutic target. In this study, the expression levels of ANGPTL4, IgM, IgG, TNF-α, IL-6 and IL-1β were investigated in TB patients (n=50) and healthy individuals using western blot and ELIZA. were measured. The expression levels of ANGPTL4, IgM, TNF-α, IL-6 and IL-1β increased significantly in patients compared to the control group (healthy group) (P < 0.05) and IgG changes were not significant. The results of our study show that the identification of specific immunological markers and their association with ANGPTL4 expression can lead to the discovery of new diagnostic biomarkers for TB. These biomarkers can be used to improve the accuracy and efficiency of TB diagnosis, especially in resource- limited settings where traditional diagnostic methods may be limited.